Kalkitoxin

Kalkitoxin, a lipopeptide derived from the cyanobacterium Lyngbya majuscula, induces NMDA receptor mediated neuronal necrosis, blocks voltage-dependent sodium channels, and induces cellular hypoxia by inhibiting the electron transport chain (ETC) complex 1. Kalkitoxin, a lipopeptide derived from the cyanobacterium Lyngbya majuscula, induces NMDA receptor mediated neuronal necrosis, blocks voltage-dependent sodium channels, and induces cellular hypoxia by inhibiting the electron transport chain (ETC) complex 1. Kalkitoxin is an ichthyotoxin, derived from the cyanobacterium Lyngbya majuscula which covers sections of the coral reef. It typically forms mini-blooms and produces several metabolites, such as kalkitoxin, curacin-A and antillatoxin. Kalkitoxin has been found and purified near the coasts of Curaçao and Puerto Rico. Kalkitoxin is a lipopeptide toxin with a molecular weight of 366.604Da. Its chemical formula is C21H38N2OS. The structure contains two double bonds, a 2,4-disubstituted thiazoline ring system, and an additional carbonyl-group. These four groups each provide a degree of unsaturation, which causes kalkitoxin to have four degrees of unsaturation. The structure contains 5 chiral centers, one of which is due to a substituent of the thiazoline ring, and the other four are due to methine groups along the aliphatic carbon chain, which are tertiary carbon atoms bearing three single carbon bonds and one hydrogen. The four methyl groups (each at a methine chiral center), the structure’s overall stereochemistry, and the N-methyl group all contribute to the toxicity of kalkitoxin. The structure of kalkitoxin was first determined by characterizing six partial structures which were subsequently connected to yield the total structure. This investigation was largely carried out through various NMR experiments. Structure (a) is a sec-butyl group, indicated by characteristic deshielding of its central methine group due to the adjacent carbonyl. Structure (b) contains this carbonyl group, and an adjacent tertiary methylated nitrogen atom, constituting a tertiary amide group. Since this is a tertiary amide, it exists in a cis/trans mixture, which underlies the two conformations of Kalkitoxin. Structure (c) is a string of two methylene groups, then a methine group bearing a high-field methyl group. The next two groups identified (d,e) are identical and opposing strings of CH2-CH-CH3, however the left grouping’s methylene protons experience greater deshielding, due to their proximity to the adjacent imine. Deshielding is an effect of a nearby electronegative atom withdrawing electron density from a given atom nucleus, eliciting an increased chemical shift as measured by NMR. The final partial structure consists of a thiazoline ring with a terminal alkene substituent, as determined by electron ionization mass spectrometry (EI-MS) and 13C NMR. The chemical shifts of ring carbons adjacent to the sulfur and nitrogen heteroatoms were compared to 13C NMR data from model compounds. This allowed for the determination of these heteroatoms' locations in the ring, and subsequently the existence of the thiazoline ring itself. With these partial structures established, their connectivity was evaluated via HMBC spectroscopy, a 2D NMR technique which allows for the determination of heteronuclear J-coupling values for nonadjacent carbons and protons. This allows for the spatial relation of specific carbon and hydrogen atoms within a structure to be determined. Kalkitoxin has five chiral centers, one of which is the ring carbon to which the terminal alkene is coordinated, with the remaining four occurring at tertiary carbon atoms along the aliphatic chain originating from the imine nitrogen. The total stereochemistry of natural (+)-kalkitoxin is 3R,7R,8S,10S,2′R. For this determination, 3JCH values by a variation of the HSQMBC pulse technique, a type of HMBC spectroscopy, and 3JHH values by exclusive correlation spectroscopy (E.COSY). These methods use NMR to evaluate the spin-spin coupling constants which directly relate to the dihedral angle of the atoms being analyzed, allowing for the determination of chirality. This was used to determine the stereochemistry of chiral centers at C7, C8, and C10. Because C7 and C8 are adjacent stereocenters, these techniques allowed for immediate determination of their relative stereochemistry, however C10 is separated from C8 by C9, which carries two diastereotopic protons. This allows for the determination of relative stereochemistry of C8 and C10 to the C9 protons through ] values, so as to relate the relative stereochemistry of C8 to C10. These methods yielded a relative stereochemistry of 7R, 8S, 10S for the aliphatic chain stereocenters. Stereochemistry at C3 was determined by Marfey's analysis, wherein the compound was ozonized and subsequently hydrolyzed to obtain cysteic acid from the thiazoline ring and attached terminal alkene. Marfey's analysis indicated this amino acid derivative was L-cysteic acid, indicating R absolute stereochemistry at C3. The absolute stereochemistry of the total molecule was determined by synthesizing the possible configurations of the already determined relative chiralities, and comparison of these to natural Kalkitoxin via 13C NMR shift differences, revealing the natural (+)-kalkitoxin stereochemistry to be 3R,7R,8S,10S,2′R. The structure-activity relationship (SAR) of a molecule is the connection between the structural moieties within the compound, and how those specific structures directly contribute to the extent and character of the molecule’s biological activity. Kalkitoxin exhibits potent cytotoxicity which relies on the complete thiazoline ring for its action. Kalkitoxin analogs lacking the complete thiazoline ring exhibit on the order of 1000-fold decreased toxicity to solid tumor cell lines. This indicates the thiazoline ring structure is a crucial component of kalkitoxin's mechanism of cytotoxicity. The necessity of the stereochemistry exhibited in natural (+)-kalkitoxin decreases moving towards the chiral centers in the core of the molecule, while the more terminal chiral centers and amide methyl group are increasingly crucial for toxicity. In a study which assayed for the toxicity of kalkitoxin and various analogs against brine shrimp, the analogs which experienced the least significant loss of potency were epimers at either C8 or C10. This indicates that C8 and C10 chiralities in natural (+)-kalkitoxin are the least critical for toxic biological activity. It is apparent that C10 chirality is less critical than C8, because the epimer of (+)-kalkitoxin at C10 is more potent than the epimer at C8. Furthermore, the removal of the C10 methyl group has a smaller impact on potency than does epimerization of C7, supporting the trend of decreased SAR correlation at core chiral centers on the aliphatic chain. Epimerization at C3, the attachment point of the terminal alkene to the thiazoline ring, further decreases potency of kalkitoxin, in agreement with the thiazoline ring and overall conformation of the leftmost segment of the molecule being critical for bioactivity. Finally, replacement of the tertiary amide with a secondary amide eliminates any observable toxicity, so this structure is crucial in the mechanism of kalkitoxin toxicity. This effort was the first total synthesis of (+)-kalkitoxin, and served the purpose of deducing the specific stereochemistry of natural kalkitoxin. This synthesis began from an alcohol bearing the proper chirality at C8 and C10 found in (+)-kalkitoxin, and carried a dimethylphenylsiloxy (DPSO) group positioned beta to C8, and a terminal alkene positioned alpha to C10. Hydroboration of this alkene gives the resulting alcohol, which is converted to an azide, which is the position at which (R)-2-methylbutyric acid is coupled to produce the sec-butyl group and amide group. The amide is subsequently methylated, finalizing the tertiary amide which has been shown to be so crucial for kalkitoxin toxicity. O-Desilylation and oxidation of the resulting alcohol produce an acceptor for a Horner-Wadsworth-Emmons reaction, wherein a carbonyl and an alpha-methylated phosphonate react to produce an alkene. In this case, a beta-keto phosphonate bearing an (R)-phenylglycine-derived auxiliary group was ligated to the molecule. This group is lost in asymmetric conjugate addition of an (R)-amino alcohol, which, through two cyclodehydration steps using Wipf’s oxazoline-thiazoline interconversion protocol, produces the thiazoline ring. The second total synthesis of (+)-kalkitoxin was only 16 steps and gave a 3% overall yield. A major aspect by which this differs from the first total synthesis of (+)-kalkitoxin is that rather than using a Horner-Wadsworth-Emmons reaction to ligate a phosphonate carrying the 4-phenyl-2-oxazolidinone, an organocopper conjugate addition reaction was used instead. This was done specifically by connecting the organocopper species to a 4-phenyl-2-oxazolidinone carrying an (S)-N-trans-crotonyl group through a 1,4-nucleophilic addition to the α,β-unsaturation of the crotonyl group. This method is advantageous because it allows for stereoselectivity of the resulting 1,3-dimethyl configuration during the larger sequential introduction of the methyl substituents at the C7, C8 and C10 chiral centers.