Evaluation of Various Silicon- and Boron-Containing Compounds for the Detection of Phosphorylation in Peptides via Gas-Phase Ion-Molecule Reactions:
2014
Gas-phase ion–molecule reactions (IMR) of various boron- and silicon-containing neutrals were investigated as a potential route for detecting phosphorylation within peptides in the negative ion mode. Trimethyl borate (TMB), triethyl borate (TEB) and N,O-Bis(trimethylsilyl)acetamide (TMSA), unlike diethylmethoxyborane (DEMB), diisopropoxymethylborane (DiPMB) and chlorotrimethylsilane (TMSCl), reacted differently if a phosphate moiety was present and thus are suitable to detect phosphorylation. During multistage collision-induced dissociation experiments of the reaction products of IMR with TMB and TEB, the [LSsF − 4H + B]− ion formed a modified y2* fragment allowing the phosphorylation site to be assigned, unlike reaction products of DEMB and DiPMB which lost both the phosphoric acid and the boron-containing moiety.
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