Determination of geniposide in Chaizhi Shugan Pills by HPLC

Objective To establish an HPLC method for the determination of geniposide in Chaizhi Shugan Pills.Methods Geniposide was separated on Zorbax SB-C18(4.6 mm×250 mm,5 μm)column,the mobile phase was acetonitrile-water(15:85) with the flow rate of 1.0 mL·min-1 and the detection wavelength was 238 nm.Results The linear range of geniposide was 0.168 6～3.372 0 μg(r=0.999 7),the average recovery was 99.3% and the RSD was 1.3%.Conclusion The method was convenient,rapid and reproducible,and it can be used for the quality control of Chaizhi Shugan Pills.