Antifungal activity of Talaromyces muroii against coffee anthracnose

Colletotrichum coffeanum causing anthranose is proved for pathogenicity to coffee var. Arabica. Talaromyces muroii EU18 is taxonomic confirmed by morphological characters and molecular phylogeny based on s-tubulin region. The crude ethyl acetate and crude methanol extracted from T. muroii EU18 showed significantly antifungal activity against C. coffeanum which the median effective dose (ED50) values for colony growth inhibition of 580.00 and 420.00 ppm, respectively. Crude hexane, ethyl acetate and methanol extracts at 1,000 ppm suppressed the colony growth of 43.00, 60.75 and 76.25 %, respectively and sporulation of 61.76, 73.02 and 80.00 %, respectively. It is clearly demonstrated that metabolites from T. muroii EU18 acts as a new antagonist against C. coffeanum causing coffee anthracnose. The chemical elucidation of bioactive compound to prove control mechanism is being done and characterized.