Development of HPTLC detection of synthetic pesticide carbosulfan in biological material

The field of agriculture is the key role in Indian society in which insecticides play a vital role in increasing the crop production yield. Carbosulfan is a member of the carbamate family; it is an important insecticide and it has a diversified role in agriculture. There is an increasing number of human poisoning cases by mainly the compound carbosulfan of the carbamate family. During the last few years, Forensic Science Laboratories of Maharashtra, India, detected a large number of human poisoning cases with carbosulfan since a large number of biological samples (postmortem sample) were received for toxicological chemical analysis. Thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) were the preferred methods of choice. By observing the results of toxicological chemical analysis, the conclusion can be drawn that carbosulfan plays a major role in poisoning cases. The chromogenic reagent was found to be a selective spray reagent for carbosulfan in routine analysis by HPTLC. Carbosulfan reacts with chromogenic reagent which gives more intense pink-colored compounds than the other organochlorine insecticides, organophosphorus insecticides, and pyrethroid insecticides. Biological material constituents (amino acids, peptides, proteins, fats, etc.) do not interfere with this chromogenic reagent.