Its Acetylation Activity Destabilizes Microtubules Independently of TAT1 α Tubulin Acetyltransferase

Paul A. Heppenstall,Daniel Bilbao-Cortés,Karol Fiedorczuk,Concepción Martínez,Emerald Perlas

Its Acetylation Activity Destabilizes Microtubules Independently of TAT1 α Tubulin Acetyltransferase

2014

Paul A. Heppenstall
Daniel Bilbao-Cortés
Karol Fiedorczuk
Concepción Martínez
Emerald Perlas

-acetyltransferasesuperfamily,hasbeenidentiﬁedasan -tubulinacetyltransferaseinciliatedorganisms.Here,weexploredthefunctionof TAT1withtheaimofun-derstandingtheconsequencesof TAT1-mediatedmicrotubuleacetylation.Wedemonstratethat -tubulinisthemajortargetof TAT1butthat TAT1alsoacetylatesitselfinaregulatorymechanismthatisrequiredforeffectivemodiﬁcationoftubulin.Wefur-thershowthatinmammaliancells, TAT1promotesmicrotubuledestabilizationandacceleratesmicrotubuledynamics.Intriguingly,thiseffectpersistsinan TAT1mutantwithnoacetyltransferaseactivity,suggestingthatinteractionof TAT1withmicrotubules,ratherthanacetylation

Keywords:

Acetyltransferase
Microtubule
Cell biology
Tubulin
Acetylation
Chemistry
α tubulin

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