Cloning and Expression of the Extracellular Protein factor of Streptococcus suis

By molecular cloning method,full-length gene Ef was cloned into the downstream of the Ptac promoter of expression vector pGEX-6p-1,electric shock transformed into E.cohi BL21(DE3)expression.After PCR amplification,and protein gel electrophoresis,the EF protein was effectively expressed,providing further construction of bio-engineering vaccine of Streptococcus suis serotype 2 with an initial vector.