Signaling via ITGB1/FAK and microfilament rearrangement mediates the internalization of Leptospira interrogans in mouse J774A.1 macrophages
2015
Leptospirosis caused by pathogenic Leptospira species is a worldwide zoonotic
2 infectious disease, but the mechanisms of leptospiral internalization
remain poorly understood. Here, we report that mouse J774A.1 macrophages
expressed integrin-subfamily proteins (ITGB1, ITGB2 and ITGB3). Antibody
blockage and siRNA-based knockdown of ITGB1 decreased the internalization of
leptospires into mouse J774A.1 macrophage cells. The internalization required
focal adhesion kinase (FAK) activation in J774A.1 cells rather than
phosphoinositide-3-kinase (PI3K), and microfilament rather than microtubule
aggregation during infection. The data indicated that the
ITGB1/FAK/microfilament signaling pathway is responsible for leptospiral
internalization in mouse macrophages.
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