Computer analysis and evaluation of ultrasound B-images universally usable due to principle of B-MODE and its displaying in grayscale

We developed an algorithm and simultaneously a computer program to analysis and evaluation of ultrasound B-images. Ultrasound B-imaging is one of mostly used displaying method in radiology. Our developed method and program use of the principle of B-imaging and displaying in grayscale according to pulse-echo approach principle. The program is focused on measurement echogenic area inside defined ROI. So, we measure level of echogenicity for examined tissue. It is based on binary thresholding due to grayscale displaying. We observe how echogenic area is changed according to threshold. Results were verified by an experienced sonographer and also by independent observers. We also observed average correlation between different ultrasound machines (r > 0.82) and between observers (r > 0.85, inter-kappa > 0.83, intra-kappa > 0.9). Up to now we tested the program for substantia nigra echogenicity to Parkinson's Disease diagnosis, raphe nucleus echogenicity and currently we examine atherosclerotic plaques in B-images with high correlation between 2 independent observers (r > 0.75) in a pilot study. Due to reached results of verification we proved the developed algorithm should be universally used in many diagnostics in which is B-MODE used, not only in neurology.