An efficient protocol for the inter-simple sequence repeat (ISSR) marker approach in population genetic studies

Molecular tools opened a new vista to understand nature’s bio-diversity and its relevance and the same approach was availed to build-up the foundation work on the bio-diversity. Screening the available biodiversity germplasms and evaluating their genetic diversity could optimize and facilitate the breeding process. ISSRs within a species can be highly variable regions of DNA.. Consequently, this technique has been used for DNA fingerprinting and assessing genetic diversity in germplasm. ISSRs are presumably noncoding loci and are dispersed throughout the genome, and their association with agronomic traits is influenced by the breeder only in regions under selection. The production of large numbers of fragments, the reproducibility, and the low costs are advantages of using ISSR markers. Thus, ISSR could be an unbiased tool for evaluating changes in diversity in agronomically important crops and genetically diverse pant and animal species. Here we devise the protocol for ISSR marker identification in relation to genetic divergence Studies.