A Wide Range of pH-Sensitive Fluorescent Probe based on Responsive Double Hydrophilic Block Copolymer for Real-Time Intracellular pH Sensing in Water Samples

In this work, a wide range of pH-sensitive fluorescent probe has been designed by the responsive double hydrophilic block copolymer (DHBCs), which containing the fluorescein (Methacrylic salicylaldoxime, SHMa) and rhodamine (Rhodamine B methacryl hydrazide, RhSAMa) derivatives in the DHBCs. The pH-sensitive fluorescent probe gave blue and red fluorescence emissions at basic pH and acidic pH, respectively. Moreover, the fluorescent probe exhibited changes in fluorescence intensity with an alteration of turbidity of the block copolymer induced by temperature. Furthermore, we have developed a model that combines with the mobile phone for quantitative and qualitative measurement of pH value. The color extract APP for real-time pH measurement in mobile phone was constructed and used to detect the pH of the water samples.