Investigation of the effects of laminin present in the basal lamina of the peripheral nervous system on axon regeneration and remyelination using the nerve acellular scaffold.

This study aimed to investigate the effects of laminin (LN) located in basal lamina, which are important components of the peripheral nervous system-extracellular matrix (PNS-ECM), on axon regeneration and remyelination. Nerve acellular scaffolds (NASs) (S-untreated) were prepared using the acellular technique. The active component LN in the NASs was blocked (S-LN(-) ) or upregulated (S-LN(+) ); S-LN+ contained seven times more LN than did the S-untreated group. The adhesion capacity of Schwann cells (SCs) to the three types of NAS (S-untreated, S-LN(-) and S-LN(+) ) was assessed in vitro. Our results showed that adhesion of SCs to the NASs was significantly reduced in the S-LN(-) group, whereas no difference was observed between the S-LN(+) and S-untreated groups. The pretreated NASs were used to repair nerves in a nerve injury mouse model with the animals divided into four groups (S-LN(-) group, S-untreated group, S-LN(+) group and auto-graft group). Two weeks after surgery, although there was no difference in the S-LN(-) group, S-untreated group and S-LN(+) group, the newly formed basal lamina in the S-LN(-) group were significantly lower than those in the other two groups. Four weeks after surgery, the S-LN(+) group had higher numbers of newly generated axons and their calibers, more myelinated fibers, thicker myelin sheaths, increased myelin basic protein expression and improved recovery of neural function compared to those of the S-LN(-) and S-untreated groups, but all of these parameters were significantly worse than those of the auto-graft group. Downregulation of the LN level in the NAS leads to a reduction in all of the above parameters. This article is protected by copyright. All rights reserved.