Process for destroying tumors and tumor associated endothelial cells using adenoviral mutants.

in vitro procedure to remove selectively and substantially, dividing cells, in a cell population containing cells in dividing and quiescent, said method comprising contacting said cell population under infective conditions with a replication-competent adenovirus comprising a mutation in the region binding to a member of RB family E1A, and let the time for said adenovirus to infect said cell population, wherein said mutation is located in the E1A-CR2 region and comprises a deletion or substitution of one or more amino acids, (a) 122 to 129; or (b) 111 to 123.