Development of Cys38 knock-out and humanized version of NbAahII10 nanobody with improved neutralization of AahII Scorpion toxin

Rahma Ben Abderrazek1, Cecile Vincke2,3, Issam Hmila1, Dirk Saerens2,3, Naima Abidi1, Mohamed El Ayeb1, Serge Muyldermans2,3 and Balkiss Bouhaouala-Zahar 1,4,5 Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, 13 Place Pasteur, BP-74, 1002 Tunis, Tunisia, Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium, Department of Cellular and Molecular Interactions, VIB, Brussels, Belgium and Faculte de Medecine de Tunis, Universite de Tunis-El Manar, Tunisia To whom correspondence should be addressed. Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, 13 Place Pasteur, BP-74, 1002 Tunis, Tunisia. E-mail: balkiss.bouhaouala@pasteur.rns.tn