Observation on variation of antibody in EgM9 vaccinated dogs.

In order to understand the immune mechanism and protective efficiency of recombinant protein EgM9, indirect ELISA method was applied to detect the variation of antibody expression in dogs vaccinated with recombinant protein EgM9. The antybody of IgG, IgG subclasses, IgM, IgA and IgE were detected, in the serum of dogs vaccinated by mixture of 100μg EgM9 and adjuvant QuilA as well as mixture of 5mg GST and adjuvant QuilA, respectively. Autopsy of vaccinated dogs were conducted on the 45th day and the number of parasite in vivo was calculated. The result showed different responses of dominant antibody IgG and IgA between vaccination and control group(0.01P0.05), while IgG1 and IgG2 exhibited very distinctly different responses between vaccination and control group(0P0.01). But IgM and IgE didn’t show any different responses between vaccination and control group. At the same time, the amount of antibody reached 40 000. Comparing with control group, the protection rate of vaccination group reached 86% after being challenged by protoscoleces (PSCs). It’s indicated that EgM9 recombinant protein could produce high antibody response, which could inhibit the development of Echinococcus granulosus and is expecting to be a candidate vaccine for Echinococcosis prevention in dogs.