A Simple Method for Rapid Preparation of Rice Genomic DNA

A simple method for preparation of rice genomic DNA was developed.A small amount(1-50mg)of leaf tissue of rice seedling,500μL of extraction buffer,and one steel bead were put into a 2-mL microcentrifuge tube.After vigorously mashing for 2min,5μL of the supernatant was directly applied to PCR amplification.Otherwise,the supernatant was precipitated with 2volumes of ethanol to obtain high quality genomic DNA.This method is simple,rapid,low cost,and reliable for PCR analysis.One person can manipulate as many as 96samples for PCR in 10minutes.It is especially suitable for genotyping of large number of samples.