IN-VITRO ANTITUMOR-ACTIVITY OF THE PLANT RIBOSOME-INACTIVATING PROTEINS MAP-30 AND GAP-31

: MAP 30 and GAP 31 are plant proteins isolated from Momordica charantia and Gelonium multiflorum, respectively. They have recently been shown to inhibit HIV-1 infection and replication. These proteins also possess a novel DNA topoisomerase-poison-like activity as well as ribosome inactivation. They were submitted to the Anti-Cancer Drug Screening Program of the National Cancer Institute and found to have potent anti-tumor activity against certain human tumor cell lines. The most sensitive cell lines responded to MAP 30 and GAP 31 with GI(50) that ranged from 0.01 to 10 mu g/ml and were unrelated to tumor type. These included cell lines from renal, non-small cell lung, and breast cancer. Targeted immunofusions made with MAP 30 or GAP 31 may be most effective toward these types of tumors.