[Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells].

The saturated free fatty acid(FFA),palmitate,could induce apoptosis in various cell types,but little is known about its effects on human umbilical cord-derived mesenchymal stem cells(hUC-MSCs).Here,we investigated whether palmitate induced apoptosis and endoplasmic reticulum(ER) stress in hUC-MSCs.hUC-MSCs were stained by labeled antibodies and identified by flow cytometry.After administration with palmitate,apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit.Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR.The mRNA of BiP,GRP94,ATF4 and CHOP were analyzed by real-time PCR.Relative BiP and CHOP protein were analyzed using Western blot analysis.The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing,BiP,GRP94,ATF4 and CHOP transcription.These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.