A study to determine the prefreeze motility parameters suggesting a favorable outcome

Introduction The recovery of functionally intact spermatozoa from thawed samples is dependent on the cryopreservation process and initial quality of the semen sample. Material and Methods 100 semen samples were cryofrozen by conventional slow freezing method. With reference of post thaw PR ≥0.4 (ratio of post thaw progressive motility/ pre thaw progressive motility) samples were grouped into poor freezability ejaculates (PFE, n = 19) with ratio < 0.4 and good freezability ejaculates PR ≥0.4 (GFE, n = 81). Results There was no statistical significance of survival of sperms with age of husband and sperm count prior to cryopreservation. The semen samples which had curvilinear velocity in the range of 56.47- 97.95 um/s-1, straight line velocity 42.811 - 72.649 um/s-1 and average path velocity 39.77- 70.11um/s-1 before freezing have good survival of sperms after thawing. Conclusions Assessment of the prefreeze velocity parameters by CASA to predict the survival of sperms post thawing so that proper counseling of patients regarding cryosurvival of sperms could be done during semen cryopreservation.