High-performance thin-layer chromatographic quantification of three active compounds from the decoction of Abnormal Savda Munziq prescription

A simple and rapid high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of rutin, rosmarinic acid, and glycyrrhizin, three active compounds from the decoction of Abnormal Savda Munziq (ASMq) prescription. The method employed silica gel GF254 thin-layer plates as the stationary phase, with ethyl acetate-formic acid-acetic acid-water (15:1:1:1.5, ½/½) as the mobile phase. Following development, plates were observed under ultraviolet (UV) light at 250 nm. The method was validated for linearity, precision, robustness, recovery, limit of detection (LOD), and limit of quantification (LOQ). Linear regression analysis data for the calibration plots showed good linear relationships (r) from 0.9992 to 0.9993. Recovery of the three compounds was between 97.86 and 100.77%. LOD and LOQ were in the range of 33.30–38.17 ng and 110.80–127.26 ng for the three compounds, respectively. The method is rapid, simple, effective, and easy-to-use for ASMq to perform routine quality cont...