Soil microbial community, C, N, and P responses to long-term tillage and crop rotation

Abstract Tillage and crop rotation/intensity can influence soil biological properties and relevant soil processes including C sequestration. This study determined the effects of long-term (25 years) no till (NT) and conventional tillage (CT) management and cropping sequence [continuous wheat (CW; Triticum aestivum L.) and a rotation of sorghum ( Sorghum bicolor L. Moench), wheat and soybean (RW; Glycine max L. Merr)] on soil microbial community structure and labile and recalcitrant microbial bio-products in central Texas. Fatty acid methyl ester (FAME) profiles, microbial biomass (MB-C, -N and -P), hot water extractable soil carbohydrates (HWE-SC) and easily extracted- (EE-) and total-glomalin-related soil proteins (T-GRSP) were analyzed. Principal component analysis of the FAME data indicated that crop management modified and selected microbial populations. In general, NT–RW resulted in the greatest richness and biodiversity of the total microbial community, soil organic C, MB-P, HWE-SC, EE- and T-GRSP. No tillage increased labile and more recalcitrant bio-products, soil organic C and total N compared to CT. The soil microbial biomass C:N:P ratio, an indicator of ecosystem nutrient limitation, suggested that the CT–RW treatment may have a soil P limitation, which was not observed in the other treatments. The treatments preferentially selected for different microbial communities, which generated microbial products that significantly influenced soil C and N retention. Our results suggested that NT in conjunction with crop rotation (RW) can be recommended for increased soil C sequestration.