Nitric oxide regulates lateral root formation through modulation of ACC oxidase activity in sunflower seedlings under salt stress

ABSTRACTNitric oxide (NO) is established as a modulator of various developmental processes in plants through its interaction with multiple enzymatic and non-enzymatic biomolecules. Lateral root (LR) induction and extension in sunflower (Helianthus annuus L.) has been observed to be governed by a probable crosstalk between NO and ethylene biosynthesizing enzyme-ACC oxidase. NaCl (120 mM) stress not only lowers LR induction but also reduces their extension growth. Quenching of endogenous NO by raising seedlings in presence of 40 µM hemoglobin in the growth medium does not affect LR induction but lowers their extension growth. NaCl stress and NO depletion have additive effects on the enhancement of ACC oxidase activity, leading to enhanced ethylene biosynthesis. Role of NO has been further confirmed by raising sunflower seedlings in the presence of 20–60 µM of two NO donors, sodium nitroprusside (SNP) and diethylenetriamine NONOate (DETA). LR extension growth was higher with DETA than SNP as NO donor at 40 µ...