[Effects of agonists of PPAR-gamma on angiotensin II-induced proliferation and extracellular matrix synthesis in hypertrophic scar fibroblasts].

Objective To study the effects of peroxisome prolilerator-activated receptor-γ agonists on angiotensin Ⅱ-induced cellular response in cultured fibroblasts derived from patients with hypertrophic scars,so as to investigate its effects on preventing the formation of hypertrophic scars.Methods Fibroblasts were freshly isolated from hypertrophic scars and cultured with angioten Ⅱ,rosiglitazone and GW9662 at a certain concentration.Fibroblasts proliferation were assessed via Cell Counting Kit-8; the mRNA and protein expressions of Collagen Ⅰ and Fibronectin (FN) were determined by quantitative realtime RT-PCR and Western blotting.Results The absorbance of CCK-8 and relative expression of Collagen Ⅰ,FN mRNA and protein were 1.082 5 + 0.007,6.45 ± 0.97,4.92 ± 0.86,2.92 ± 0.41,2.78 ± 1.04 in Ang Ⅱ group;0.7224±0.012,1.82±0.34,1.78±0.27,1.57±0.46,1.68+0.39 in Ros + Ang Ⅱ group; 0.554 7 ±0.012,0.97 ±0.12,1.07 ± 1.08,1.05 ±0.43,1.14 ±0.36 in Ros group; 1.056 0 ±0.005,5.83 ±0.24,4.47 ±0.32,2.69 ±0.35,2.62 ±0.27 in GW9662 + ros + Ang Ⅱ group.The results showed a significant difference between the Ang Ⅱ group and the control group(P ＜ 0.05).The effect of Ang Ⅱ could be markedly inhibited by Ros (P ＜ 0.05).In addition,Ros did not influence cell proliferation and production of extracellular matrix(P ＞ 0.05).There was a significant difference between the GW9662 + Ros + Ang Ⅱ group and the Ros + Ang Ⅱ (P ＜0.05).Conclusions PPAR-γ agonists inhibit Ang Ⅱ-induced proliferation and extracellular matrix synthesis effectively in the hypertrophic scar fibroblasts.Thus PPAR-γ agonists may have potential therapeutic effect for hypertrophic scar. Key words: Hypertrophic scar;  Peroxisome prolilerator-activated receptor γ;  Angiotensin Ⅱ ; Fibroblasts