A Method of Genomic DNA Extraction of Maize

Some molecular technologies including SSR (Simple Sequence Repeat) have been important methods for maize traditional breeding and the process needs analysis of a large individual samples. So the method for DNA extraction with rapid and efficient is essential. In this study, young leaves per plant of high oil maize backcross generation groups 25-30 d as the material, the establishment of EDTA, CTAB, KCl as the main reagent, and tissue grinding rod mill in liquid nitrogen as the main steps of the rapid extraction of genomic DNA, discussed the possibility for DNA which be extracted used in SSR marker-assisted selection.