Concatenation cDNA sequencing for transcriptome analysis

Abstract We describe a high-throughput cDNA sequencing pipeline ( http://www.hgsc.bcm.tmc.edu/projects/cdna ) built in response to the emerging need for rapid sequencing of large cDNA collections. Using this strategy cDNA inserts are purified and joined through concatenation into large molecules. These ‘pseudo-BACs’ are subjected to random shotgun sequencing whereby the majority of cDNA inserts in the pool are sequenced. Using this concatenation cDNA sequencing platform, we have contributed more than 13 000 full-length cDNA sequences from human and mouse to the Mammalian Gene Collection (MGC). To cite this article: P.H. Gunaratne et al., C. R. Biologies 326 (2003).