Blood and Liver Antioxidant Capacity of Mice Fed High Fat Diet Supplemented with Digested Oat Bran Proteins

The aim of this study was to assess the effect of three concentrations of oat bran protein hydrolysate (OPH) (1, 10, 10 mg/g diet) on oxidative stress marker in high fat fed animals. CD-1 male mice were placed into five groups and fed normal diet (ND), high fat (HF), and HF containing 1, 10, and 100 mg OPH/g of HF-diet for 3 weeks. Blood was collected at necropsy and analyze for glucose and markers of oxidative stress. At the highest level of OPH the oxygen radical absorbance capacity value of erythrocytes (123.1 ± 11.1 mMTrolox equivalents (TE)/mL blood) was higher (p < 0.05) than the value of HF group (96.5 ± 6.6 mM TE/mL) indicating higher scavenging activity that may be explained by higher thiol concentration detected. Liver superoxide dismutase (SOD) antioxidant enzyme activity was 13.2% lower in mice on HF-diet compared to those who received normal diet. Supplementation of HF-diet with OPH increased SOD activity to ND group level. OPH also had positive effect on respiratory exchange ratios but did not affect liver scavenging activity, calorie intake or bodyweight.In conclusion, addition OPH to HF diet increased radical scavenging activity in erythrocytes and SOD activity in mice liver samples.