The dynamic surface properties of green fluorescent protein and its mixtures with poly(N,N-diallyl-N-hexyl-N-methylammonium chloride)

Abstract Measurements of the dynamic surface elasticity and surface pressure of solutions of green fluorescent protein (GFP) indicate the formation of a dense protein monolayer with a relatively high surface elasticity of approximately 65 mN/m, and are typical for solutions of globular proteins. The mixed solutions of GFP and poly(N,N-diallyl-N-hexyl-N-methylammonium chloride) (PDAHMAC) demonstrate more complex behavior. During the first adsorption step the surface properties are determined by an unbound component, while the next step is mainly the adsorption of the complexes. The dynamic surface elasticity at relatively low polyelectrolyte concentrations (