Nucleic acid detection method for separating light-emitting marker based on magnetic bead and hydrolysis of polysaccharide

The invention discloses a nucleic acid detection method for separating a light-emitting marker based on a magnetic bead and hydrolysis of polysaccharide. The method comprises the following steps: modifying a polysaccharide molecule which is suitable for being connected with a nucleic acid probe on the surface of the magnetic bead; designing a functional probe for detecting a target nucleic acid fragment and modifying the functional probe on the magnetic bead surface which is combined with the polysaccharide molecule; capturing the target nucleic acid fragment and the light-emitting marker thereof on the surface of the magnetic bead by hybridization; magnetically separation and washing to obtain the magnetic bead of which the light-emitting marker is obtained; adding a polysaccharide hydrolysis reagent; separating the light-emitting marker from the surface of the magnetic bead by hydrolysis of the polysaccharide to obtain a light-emitting marker solution containing no magnetic bead; performing light emission detection on the solution to obtain information of target nucleic acid. The method disclosed by the invention can be used for totally overcoming light emission signal loss caused by shielding effect of the magnetic bead to a light emission signal and detecting at least 50fM of nucleic acid fragments, and is high in detection sensitivity.