Resonance Assignment of ~3C-lSN-Labeled Snake Neurotoxin II from Naja oxiana

Neurotoxin II from Naja oxiana venom is a short-chain snake curaremimetic neurotoxin containing four disulfide bonds. We obtained ~3C-~SN-labeled neurotoxin II to study its intemal dy- namics and surface properties with atomic resolution. The recombinant protein has the native spatial structure and is biologically active. The nearly complete assignment of ~H, ~3C and ~SN resonances for neurotoxin II was obtained by heteronuclear triple-resonance nuclear magnetic resonance spec- troscopy. Analysis of the secondary chemical shifts of the ~H ~, ~3C~, ~3C~ and ~3CO nuclei reveal their strong correlation with the protein secondary structure.