Antioxidant Activity of Litsea petiolata Hk. f

Background. The antioxidant activity of the extract and fractions of the Litsea petiolata Hk. f stem bark was studied in two different assay systems: DPPH assay and FRAP assay. Methods. Extract was obtain by maceration used dichloromethane as solvent. The fractionation used column chromatography with gradient solvent system. The antioxidant test used DPPH assay and FRAP assay. Results . The IC50 values for the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test of the dichloromethane extract was 27.36 µg/ml, the fraction B was 113.74  µg/ml, fraction D was 60.17 µg/ml, and the control positive (quercetin) was 3.96 µg/ml. The EC50 values for ferric ion reducing antioxidant potential (FRAP) test of the dichloromethane extract was 13.47 µg/ml, the fraction B was 76.49  µg/ml, fraction D was 55.73 µg/ml, and the control positive (quercetin) was 14.01 µg/ml. Conclusion. The extract showed higher antioxidant activity than the fractions and by FRAP test the extract showed better antioxidant activity than the positive control (quercetin).