Effects of Valsartan on the expression of megsin in glomerular mesangial cells under high concentration glucose circumstance

This study aimed to observe the effects of Valsartan on the expression of megsin,p38MAPK signaling pathway,monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) in mouse glomerular mesangial cells under high concentration glucose circumstance and investigate the role of Valsartan in the pathogenesis of diabetic nephropathy.We cultured mouse glomerular mesangial cells and the cell proliferation was measured by MTT at 12,24 and 48 h respectively after culture.The expressions of megsin,p-p38MAPK,MCP-1 and ICAM-1 in mesangial cells were detected by immunocytochemical staining and Western blot,while the concentration of type Ⅳ collagen in supernatant of mesangial cells was measured by ELISA.We found that under high concentration glucose circumstance,the expression of megsin,p-p38MAPK,MCP-1,and ICAM-1 were increased,the concentration of type Ⅳ collagen in the cellular supernatant was increased,and mesangial cell proliferation was enhanced compared with control group(P 0.05).After treatment with Valsartan,the above changes were weakened.Our results suggested that Valsartan can down-regulate the expressions of megsin,p-p38MAPK,MCP-1 and ICAM-1,even suppress mesangial cell proliferation and mesangial extracellular matrix accumulation,so that play a key role in renal protection beyond down-regulate blood pressure.