Determination of 5-Hydroxymethylfurfural in Ribavirin and Glucose Injection by HPLC

OBJECTIVE To establish an HPLC method for the determination of 5-hydroxymethylfurfural(5-HMF) in ribavirin and glucose injection.METHODS The HPLC method was performed on a Diamonsil C18 column(250 mm×4.6 mm,5 μm) with 284 nm as the detection wavelength,and methanol-water(30:70) was used as mobile phase.The column temperature was room temperature,and the flow rate was 1.0 mL·min-1.RESULTS Ribavirin and excipients did not interfere with the determination of 5-HMF.The regression results which was based on weighted least square method showed a good linearity within the range of 0.099 4-7.949 μg·mL-1 and 0.002 0-0.198 7 μg·mL-1(r=0.999 0 and 0.999 8,respectively) for 5-HMF.The LOQ and LOD of 5-HMF were 0.04 and 0.012 ng,respectively,and the average recovery was 100.4% with RSD of 1.8%(n=6).CONCLUSION The HPLC method was accurate,sensitive and suitable for determining the content of 5-HMF in ribavirin and glucose injection.