Assessment of green regenerants from rye and triticale anther cultures

Doubled haploids (DH) are a potential tool for plant breeding, gene technology and basic research. With self pollinated species, the possibility to fix a homozygote in an early generation facilitates variety breeding directly (Snape 1986). The prerequisites for using DH-technology are that a) the production method can be applied to a large number of genotypes, b) large numbers of green DH regenerants can be produced at reasonable cost and c) the green regenerants are of high quality, displaying the genetic variability of the cross. The methods for producing DH include anther and isolated microspore culture, and ovary culture after pollination with alien pollen. With cereals, success in anther culture still depends largely on the genotype despite methodological developments to circumvent this.