Production of purified somatostatin in E. coli cells comprises expression in the cells of the coding gene cloned in the vector pGRV1, with digestion by cyanogen bromide for chromatographic purification (Machine-translation by Google Translate, not legally binding)

Producing and purifying somatostatin in Escherichia colicells using isopropyl-beta-D-thiogalactopyranoside (IPTG) inducer molecule, based on the expression of a gene encoding for somatostatin pGRV1 cloned in a plasmid, comprises: (a) cloning the somatostatin gene in the plasmid pGRV1 to obtain pSOMA vector (b) transforming in E. colistrain BL21-DE3 with plasmid pSOMA, to obtain the strain CECT5840 (c) fermenting strain CECT5840 and (d) extracting protein, permeabilizing inclusion bodies, and chromatographic purification to obtain somatostatin. Producing and purifying somatostatin in E. colicells using isopropyl-beta-D-thiogalactopyranoside (IPTG) inducer molecule, based on the expression of a gene encoding for somatostatin pGRV1 cloned in a plasmid, comprises: (a) designing a gene encoding human somatostatin comprising a fully defined 54 bp sequence (SEQ ID NO. 2) given in the specification (b) constructing plasmid pGRV1 comprising a fully defined 6343 bp sequence (SEQ ID NO. 8) given in the specification (c) cloning the somatostatin gene in the plasmid pGRV1 to obtain pSOMA vector (d) transforming in E. colistrain BL21-DE3 with plasmid pSOMA, to obtain the strain CECT5840 (e) fermenting strain CECT5840 in SL media under controlled conditions and (f) extracting protein, permeabilizing inclusion bodies, and chromatographic purification to obtain somatostatin. (Machine-translation by Google Translate, not legally binding)