Estimation of auxins and cytokinins requirements in sugarcane soma clones for effective in vitro regeneration procedure

Three sugarcane varieties were used in this experiment to optimize specific plant hormones. The present work was conducted in 2014 at Nuclear Institute of Agriculture (NIA), Tando jam. The experiment was designated with three sugarcane varieties (NIA-2012, NIA-105 and GULABI-95) obtained from Nuclear Institute of Agriculture (NIA) Tando jam. Regeneration of plantlets was compared under different concentration of auxins and cytokinin (IAA, IBA and kinetin (2.0, 3.0 mg1-1), highly significant (p<0.05) variations were observed for all parameters of regeneration and root formation. Interactive effect of variety x treatment x concentration was non-significant for number of regenerated plantlets. Auxins and cytokinins at 3.0 + 3.0 mg1-1 concentration were most optimized and effective for regenerated plantlets and number of shoots. These concentrations should be used in the future for in vitro culture of sugarcane. Key words: In vitro, regeneration, cytokinins, sugarcane, growth regulators.