Preconcentration of codeine in pharmaceutical and human urine samples by multi-walled carbon nanotubes and its spectrophotometric determination

A rapid and efficient solid phase extraction method was established for the preconcentration of codeine prior to its simple determination by spectrophotometry. The extraction process is based on the multi-walled carbon nanotubes (CNTs) containing –COOH functional groups. These CNTs showed a great affinity for the low quantities of codeine in certain condition. Various extraction factors including buffer type, concentration and its pH, ionic buffer, incubation time, and eluent were optimized to achieve high sensitivity. The calibration graph was linear in the codeine concentration range of 0.001–4 mg·L−1, with a detection limit (3s) of 0.4 μg·L−1. The relative standard deviation (RSD %) for the repetitive determination of 0.01, 0.5, and 2 mg·L−1 codeine (n = 5) were 1.56%, 2.01%, and 1.63%, respectively. Furthermore, comparison with other reported methods showed that the presented method has suitable characteristics. Finally the method was successfully used to accurately determine codeine in pharmaceutical...