Expression Profiles of Glutathione S-Transferase Genes in Bombyx mori Exposed to NaF

In order to explore the roles of Bombyx mori glutathione S-transferase gene (BmGST) in detoxification and resistance to insecticides, we used real-time PCR method to detect the transcription levels of five BmGST genes in different tissues of the 5th instar larvae feeding on sodium fluoride treated mulberry leaves. The detection results were normalized by using 3 internal reference genes. The results indicated that the transcription levels of BmGSTs were different in various tissues. Transcription of BmGST genes could be induced by NaF, The normalized data with the above 3 internal reference genes indicated that, it is very important to choose adequate internal reference genes so as to ensure the reliability of the detection results.