Study on the Polymorphisms of LALBA Gene 5′ Regulation Region in Cattle

In order to analyze the polymorphisms of LALBAgene 5′regulation region in cattle.Two cattle breeds(Wuchuan Black cattle and Guizhou Holstein cow)with significant difference in breeds property were selected to construct DNA pools,a total of 1126bp sequences of LALBA5′regulation region and part of exon 1were obtained by designing 1pair specific primers.The results showed that 5single nucleotide polymorphisms(SNPs)were found in the 5′regulation region which included T-114C,C-166T,A-225C,C-344T,T-778Crespectively and T-114C was specific to Wuchuan Black cattle.Furthermore,bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites.It demonstrated that 5new transcription factors binding sites emerged while 11previous transcription factors binding sites disappeared which included one located in the core region of promoter results from the SNPs found in this study,the remarkable change of secondary structure of RNA was also detected but the CpG islands was not found by using various softwares.