Salvionate protects cardiac function after cariopulmonary bypass

Objective To study the major molecular mechanism of myocytes autophagy underlying cardiac hypofunction and the effect of salvionate on cardiac function after cardiopulmonary bypass(CPB).Methods Fifteen healthy male Chinese pigs were randomly divided into CPB group(n=6),salvionate treatment group(n=6) and control group(n=3).Their cardiac function,hemodynamic parameters and inflammatory factors were measured,and apoptosis index and ultrastructure of myocardium and glomeruli were assayed by immunohistochemistry.Myocytes autophagy pathway-related signals were detected by Western blot.Results The serum IL-1β,TNF-α and IL-10 levels were significantly higher whereas the cardiac function level was lower in CPB group than in control group 2 h after CPB.The serum IL-1β,IL-2,and TNF-α levels were significantly lower in salvionate treatment group than in CPB group 2 h after CPB.The activity of mTOR signal pathway and the LC3-Ⅱ/LC3-Ⅰ ratio were significantly higher in CPB group than in control group whereas the activity of mTOR signal pathway was significantly higher while the LC3-Ⅱ/LC3-Ⅰ ratio was significantly lower in salvionate treatment group than in CPB group 2 h after CPB.Conclusion Salvionate can protect cardiac function after CPB,which is related with its involvement in inhibiting AMP kinase activity and autophagy of myocytes through the mTOR signal pathway,as well as its anti-inflammation and anti-oxidation stress effects.