Granulocyte specific cytotoxic antibodies in pregnancy and multitransfused sera

The myeloid cell line K562 was used to screen for specific antibodies in sera obtained from pregnant women and multitransfused patients. The alloantisera thus procured reacted with random granulocytes in a pattern suggestive of a polymorphic system. Some antibodies were found to react only in the cold. A simple reproducible method for the simultaneous separation of granulocytes and lymphocytes for microcytotoxicity is described.