Reversible Activation of Lysosomes by Dimethyl Sulphoxide

WE have demonstrated that dimethyl sulphoxide (DMSO), used in concentrations which protect cells against damage caused by freezing and subsequent thawing, can increase the activity of rat liver lysosomes, in vitro, as measured by increased activity of acid phosphatase1. The presence of DMSO in sufficient concentration apparently increases the permeability of rat liver lysosomes to the sodium-beta-glycerophosphate used as substrate, thereby increasing the activity of the enzyme, which was originally masked or latent. Our previous experiments also indicated that DMSO, despite releasing some acid phosphatase from lysosomes in the conditions used, might not permanently alter the lysosomal membrane.