Estrogen-Initiated Transformation of ProstateEpithelium Derived from Normal Human ProstateStem-Progenitor Cells
2012
The present study sought to determine whether estrogens with testosterone support are sufficient to transform the normal human prostate epithelium and promote progression to invasive adenocarcinoma
using a novel chimeric prostate model. Adult prostate stem/early progenitor cells
were isolated from normalhumanprostates through prostasphere formation in three-dimensional culture. The stem/early progenitor cell status and clonality of prostasphere cells was confirmed by
immunocytochemistry and Hoechst staining. Normal prostate progenitor cells were found to express estrogen receptor , estrogen receptor , and G protein-coupled receptor 30 mRNA and protein and were responsive to 1 nM estradiol-17 with increased numbers and prostasphere size, implicating them as direct estrogen targets. Recombinants of humanprostate progenitor cells with
rat urogenital sinus mesenchyme formed chimeric prostate tissue in vivo under the renal capsule of nude mice. Cytodifferentiation of human prostate progenitor cells in chimeric tissues was confirmed by immunohistochemistry using epithelial cell markers (p63, cytokeratin 8/18, and androgen receptor), whereas human origin and functional differentiation were confirmed by expression of human nuclear antigen and prostate-specific antigen, respectively. Once mature tissues
formed, the hosts were exposed to elevated testosterone and estradiol-17 for 1–4 months,
and prostate pathology was longitudinally monitored. Induction of prostate cancer in the human stem/progenitor cell-generated prostatic tissue was observed over time, progressing from normal histology to epithelial hyperplasia, prostate intraepithelial neoplasia, and prostate
cancer with local renal invasion. These findings provide the first direct evidence that human prostate progenitor cells are estrogen targets and that estradiol in an androgen-supported milieu is a carcinogen for human prostate epithelium. (Endocrinology 152: 2150–2163, 2011)
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