Somatic cell mapping of the human cyclophilin B gene (PPIB) to chromosome 15

The polymerase chain reaction (PCR) technique was used to generate a unique probe complementary to the hydrophobic 5’ end of the human cyclophilin B gene. This unique probe was hybridized to DNAs from human × hamster hybrid somatic cell lines retaining different combinations of human chromosomes. The gene was assigned to chromosome 15.