Recombinant pichia pastoris engineering bacteria and production method thereof

The invention discloses recombinant pichia pastoris engineering bacteria. The recombinant pichia pastoris engineering bacteria comprises a codon-optimized Richter trichoderma xylanase gene, and the nucleotide sequence of the gene is shown in SEQ:1 in a sequence table. The invention also discloses a production method of the recombinant pichia pastoris engineering bacteria. The production method comprises the following steps: (1) performing synonymous mutation on the Richter trichoderma xylanase gene according to pichia pastoris flavored codons so that the Richter trichoderma xylanase gene is completely mutated into the pichia pastoris favored codons; (2) synthesizing an optimized xylanase gene by utilizing a total-gene synthesis technology, and connecting the optimized xylanase gene with a pMD20-T vector to build a cloning vector pMD20T-Xynopti; (3) building a recombinant expression vector pPICZalpha-Xynopti; (4) transforming a pichia pastoris host; (5) performing induction culture of a positive converter; (6) producing xylanase by small-scale fermentation by adopting the recombinant pichia pastoris engineering bacteria. The recombinant pichia pastoris engineering bacteria disclosed by the invention can be used for secreting a great amount of xylanase.