Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection
2017
: Methylation-specific polymerase chain reaction (MS-PCR) is a more rapid way to detect changes in DNA methylation than is bisulfite sequencing. In addition, by incorporating some basic automation, samples can be prepared and analyzed in a 96-well plate format. The method can be used either quantitatively (qRT-PCR-based MethyLight) or qualitatively (using agarose gels) to detect changes in DNA methylation; both are described in this protocol.
Keywords:
- Genetics
- Polymerase chain reaction optimization
- Reverse transcription polymerase chain reaction
- Molecular biology
- Inverse polymerase chain reaction
- Biology
- DNA methylation
- Primer dimer
- Nested polymerase chain reaction
- Hot start PCR
- Real-time polymerase chain reaction
- Bisulfite sequencing
- Multiplex polymerase chain reaction
- Multiple displacement amplification
- Correction
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- Cite
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