Design and preparation of antimicrobial meat wrapping nanopaper with bacterial cellulose and postbiotics of lactic acid bacteria

Abstract Lyophilized postbiotics of Lactobacillus plantarum was prepared and impregnated in bacterial nanocellulose (BNC) by ex-situ method to develop an antimicrobial ground meat wrapping nanopaper. The postbiotics incorporated BNC (P-BNC) films were optimized by response surface methodology and their antimicrobial activity against Listeria monocytogenes were examined. The BNC with postbiotics at 21.21% concentration and 28 min impregnation time was chosen as an optimized P-BNC film. The FTIR results confirmed the immobilization of postbiotics in BNC. The P-BNC film represented a significant reduction (~5 log cycles) in L. monocytogenes counts in ground meat at the end of the storage period (9 days at 4 °C). Meat wrapped by P-BNC film displayed a significant decrease in total mesophilic and psychrophiles count and TBA values than the controls. BNC can be considered as a proper carrier for development of antimicrobial film using postbiotics of LAB for food application.