Study on EST-SSR Markers Related to Thermotolerance of Eggplant

The heat-stress related ESTs from the suppression subtractive hybridization cDNA(SSH-cDNA) library of heat-stressed eggplant and the genome database of NCBI website were analyzed and the heat-stress related EST-SSRs in eggplant were studied.9 SSR loci were found in the ESTs of eggplant heat-stressed SSH-cDNA library,the other 25 SSR loci were found in the genome database,a total of 32 pairs of SSR primers were designed.Further primer screening results indicated that the EG6 primers which targeted library EST YP249 showed polymorphism in different eggplant materials.The YP249 was then proved as an up-Regulated expression sequence in heat-stressed materials by real-time fluorescence,therefore,the EG6 amplification site was considered as a candidate SSR polymorphism locus which related to the thermotolerance of eggplant.