Abstract 3289: Elevation of oxidative expression proteins in human ovarian epithelial carcinoma

Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Oxidative stress, which results from an imbalance between ROS and antioxidant capacities, can cause a wide range of direct or indirect DNA damage to ovarian epithelium mucosa that is exposed to immense physiological changes, driven by cyclically altering hormone levels as a result of ovulation. The influence of ovulation contributed to the cause of increased oxidative stress in ovarian cells. In this study, we monitored and compared the level of oxidized carbonyl proteins in two subtypes of epithelial ovarian cancer, Serous and endometriod with normal and normal surrounding tissue. We applied DNPH derivative colorimetric and immuneblot assay, two dimension gel electrophoresis (2-D SDS-PAGE), to identify and evaluate the level of oxidative modified protein in 100 snap-freeze epithelial ovarian tissues including, normal, normal surrounding, cystoadenoma, borderline and carcinoma with age range of 65±12, (56% Caucasian 44% Africa American. Our results showed that the index of carbonyl proteins /total proteins increased by onset and progression in both serous and endometriod ovarian malignancy. The elevation of oxidized proteins among African American was significantly higher than Caucasian in advanced stages of disease. SDS-PAGE profiles and western blot analysis of oxidized proteins showed a distinct protein expression pattern among cancerous and non-cancerous tissues. Our study suggests that the level of oxidative stress may be involved in the selective modification of some enzymes and structural proteins and could play a role in etiological differences that may exist between stages of benign and invasive epithelial ovarian tumors. Citation Format: Sharifeh Mehrabi, Shakeria Cohen, Felix O. Aikhionbare. Elevation of oxidative expression proteins in human ovarian epithelial carcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3289. doi:10.1158/1538-7445.AM2014-3289