Keywords Biocompatibility, Biodentine, cytotoxicity, Sulforhodamine B assay ,Glass ionomer cement , Human Gingival Fibroblast. Comparison of Cytotoxicity of Biodentine Retrograde Filling Material Against Mineral Trioxide Aggregate and Glass Ionomer Cement- an Invitro Study.

AIM OF THE STUDY : To compare the cytotoxicity of potential retrograde filling materials against on Hu- man Gingival Fibroblasts cell line by means of the Sulforhodamine B assay . MATERIALS AND METHODS : 9 samples each of Biodentine , Mineral trioxide aggregate Angelus and Glass ionomer cement Type IX were prepared for the study and set at 37 0C in 100% relative humidity for one day . The set materi- als were immersed in Dulbecco Modified Eagle culture medium for 24 hrs. Fibroblasts cultured in Dulbecco Modified Eagle Medium were used as a control group. The test materials extracts were then separated and then tested in culture wells in close proximity to growing cell culture and incubated for 24 hrs & 48 hrs. . Cytotoxicity/ Survival fraction was es- timated by Sulforhodamine B assay. One way ANOVA test , Bonferroni test and Paired t test were used to evaluate the statistical significance of the results. RESULTS : Cells exposed to extracts from MTA Angelus and Biodentine showed the highest survival fraction percent- age after 24 hrs & 48 hrs at all elute concentrations , whereas cells exposed to Glass-ionomer cement type IX extracts displayed the lowest survival fraction percentage. CONCLUSION: On serial dilution of cement extracts , Survival fraction percentage continued to increase on further di- lution and showed better results for cell viability and compatibility of all the root filling materials tested. The degree of cytotoxicity in descending order was Glass-ionomer cement type IX, MTA Angelus & Biodentine in the cell line tested for both 24hr and 48 hrs exposure period of the study