Expression of enoyl CoA hydratase 1 reduces cell proliferation and migration in mouse hepatocarcinoma cells

Objective To study the expression of enoyl CoA hydratase 1 ( ECH1 ) and the effect when down-regulation of ECH1 gene expression in mouse hepatocarcinoma cell. Methods Immunofluorescence was used for detecting the expression of ECH1,and stably transfected Hca-F cells with pGPU6/GFP/Neo-shRNA-ECH1 expression plasmids.Cell proliferation was assessed by Cell counting kit-8(CCK8) assay.The Boyden-transwell assay (8 μm pore size) was performed to analyze the inhibitory effect of shRNA on Hca-F cell migration and invasion.Results ECH1 expression was obtained in the cytoplasm and upregulated expression in Hca-F cells than that in Hca-P cells.The down-regulation of ECH1 could inhibit the cell proliferation of Hca-F cells,decrease the number of cell pass through Transwell (27.07 ±17.49) compared with scramble-negative (72.38 ± 18.83 ) and Hca-F controls ( 59.06 ± 30.33 ),decease the migration capacities of Hca-F cells,increase the ratio of Hca-F cells in S phase ( 86.1％ ) compared with scramble-negative ( 75.8％ ) and Hca-F controls ( 66.2％ ) and decrease the ratio of G1 phase (9.4％) compared with scramble-negative (24.2％ ) and Hca-F controls (30.3％ ).Conclusion ECH1 serves as a potential critical factor attributes to tumor lymphatic metastasis. Key words: Liver neoplasms ;  Enoyl-CoA hydratase ;  Microscopy fluorescence;  Lymphaticmetastasis